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cell culture human nsclc hcc827 cell line (ATCC)

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ATCC cell culture human nsclc hcc827 cell line
Cell Culture Human Nsclc Hcc827 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 2003 article reviews

cell culture human nsclc hcc827 cell line - by Bioz Stars, 2026-08

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Cell viability of H1975OR (a), HCC827OR (b), HCC827GR (c), HCC827ER (d), and their corresponding parental cells was determined by CCK-8 assay after cells were treated with the indicated concentrations of TKIs. e–g NRF2 protein levels were detected in resistant cells compared with their parental cells by Western blotting analysis. h NRF2 protein levels were detected by Western blotting analysis in ALDH1A1-overexpressing stem-like cells after treatment with DOX for 48 h or 72 h. i, j NRF2 protein levels were detected by Western blotting analysis in HCC827 and H1975 cells after treatment with 1 μM erlotinib, gefitinib or osimertinib for 0, 24, 48, and 72 h. k NRF2 mRNA levels of HCC827, HCC827GR, HCC827ER, and HCC827OR cells were detected by RT-PCR. (l) Parental and resistant cells were treated with 100 μg/mL CHX, collected at the indicated times, lysed and immunoblotted.

Journal: Acta Pharmacologica Sinica

Article Title: NRF2-GPX4/SOD2 axis imparts resistance to EGFR-tyrosine kinase inhibitors in non-small-cell lung cancer cells

doi: 10.1038/s41401-020-0443-1

Figure Lengend Snippet: Cell viability of H1975OR (a), HCC827OR (b), HCC827GR (c), HCC827ER (d), and their corresponding parental cells was determined by CCK-8 assay after cells were treated with the indicated concentrations of TKIs. e–g NRF2 protein levels were detected in resistant cells compared with their parental cells by Western blotting analysis. h NRF2 protein levels were detected by Western blotting analysis in ALDH1A1-overexpressing stem-like cells after treatment with DOX for 48 h or 72 h. i, j NRF2 protein levels were detected by Western blotting analysis in HCC827 and H1975 cells after treatment with 1 μM erlotinib, gefitinib or osimertinib for 0, 24, 48, and 72 h. k NRF2 mRNA levels of HCC827, HCC827GR, HCC827ER, and HCC827OR cells were detected by RT-PCR. (l) Parental and resistant cells were treated with 100 μg/mL CHX, collected at the indicated times, lysed and immunoblotted.

Article Snippet: Cell lines and cell culture Human NSCLC cell lines HCC827 and PC9, which harbor EGFR mutations and are sensitive to first- and third-generation EGFR TKIs, were obtained from the American Type Culture Collection (ATCC) and Dr. G. L. Zhuang (China State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, Shanghai, China), respectively.

Techniques: CCK-8 Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction

a HCC827 cells and HCC827ER cells were transfected with 20 nM siNRF2 or mock control for 72 h, and photographs of cells were captured by IncuCyte. Scale bar: 100 µm. b–d HCC827 cells, HCC827OR cells, and HCC827GR cells were transfected with 20 nM siNRF2 or mock control for 72 h, and cell growth curves were recorded by IncuCyte. e Cell viability was measured by CCK-8 assay as described in “Methods” after HCC827 cells, HCC827ER cells, and HCC827GR cells were transfected with 20 nM siNRF2 or mock control for 72 h. f Colony forming assay was performed as described in “Methods” after cells were transfected with 20 nM siNRF2 or mock control for 48 h. g Cell death was detected by flow cytometry after HCC827GR cells were transfected with 20 nM siNRF2 or mock control for 72 h. h HCC827 and HCC827GR cells were treated with 10 μM NRF2 inhibitor ML385 for 72 h, and cell growth curves were recorded by IncuCyte. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Ctr.

Journal: Acta Pharmacologica Sinica

Article Title: NRF2-GPX4/SOD2 axis imparts resistance to EGFR-tyrosine kinase inhibitors in non-small-cell lung cancer cells

doi: 10.1038/s41401-020-0443-1

Figure Lengend Snippet: a HCC827 cells and HCC827ER cells were transfected with 20 nM siNRF2 or mock control for 72 h, and photographs of cells were captured by IncuCyte. Scale bar: 100 µm. b–d HCC827 cells, HCC827OR cells, and HCC827GR cells were transfected with 20 nM siNRF2 or mock control for 72 h, and cell growth curves were recorded by IncuCyte. e Cell viability was measured by CCK-8 assay as described in “Methods” after HCC827 cells, HCC827ER cells, and HCC827GR cells were transfected with 20 nM siNRF2 or mock control for 72 h. f Colony forming assay was performed as described in “Methods” after cells were transfected with 20 nM siNRF2 or mock control for 48 h. g Cell death was detected by flow cytometry after HCC827GR cells were transfected with 20 nM siNRF2 or mock control for 72 h. h HCC827 and HCC827GR cells were treated with 10 μM NRF2 inhibitor ML385 for 72 h, and cell growth curves were recorded by IncuCyte. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Ctr.

Techniques: Transfection, Control, CCK-8 Assay, Flow Cytometry

a Morphological phase-contrast images of cells after treatment with 20 nM siNRF2 for 48 h. b Western blot analysis of EMT biomarkers and CSC biomarker CD44 in HCC827, HCC827GR, and HCC827ER cells. c Western blot analysis of EMT biomarkers and CSC biomarker ALDH1A1 in HCC827ER, HCC827GR, and HCC827OR cells after cells were transfected with 20 nM siNRF2 or mock control for 72 h. d Cell migration ability was determined by Transwell migration assay after cells were transfected with 20 nM siNRF2 or mock control for 72 h. The quantification result was determined as described in the “Methods”. Scale bar: 100 µm. Data are presented as the mean ± SEM. *P < 0.05 vs mock, #P < 0.05 vs HCC827.

Journal: Acta Pharmacologica Sinica

Article Title: NRF2-GPX4/SOD2 axis imparts resistance to EGFR-tyrosine kinase inhibitors in non-small-cell lung cancer cells

doi: 10.1038/s41401-020-0443-1

Figure Lengend Snippet: a Morphological phase-contrast images of cells after treatment with 20 nM siNRF2 for 48 h. b Western blot analysis of EMT biomarkers and CSC biomarker CD44 in HCC827, HCC827GR, and HCC827ER cells. c Western blot analysis of EMT biomarkers and CSC biomarker ALDH1A1 in HCC827ER, HCC827GR, and HCC827OR cells after cells were transfected with 20 nM siNRF2 or mock control for 72 h. d Cell migration ability was determined by Transwell migration assay after cells were transfected with 20 nM siNRF2 or mock control for 72 h. The quantification result was determined as described in the “Methods”. Scale bar: 100 µm. Data are presented as the mean ± SEM. *P < 0.05 vs mock, #P < 0.05 vs HCC827.

Techniques: Western Blot, Biomarker Discovery, Transfection, Control, Migration, Transwell Migration Assay

a, b Cell death was determined by flow cytometry as described in the “Methods”. The cells were exposed to 100 μM DFO for 24 h. (c) ROS were analyzed by flow cytometry as described in the “Methods”. d Western blot analysis of the anti-apoptosis protein BCL2 and the DNA damage biomarker γH2AX in HCC827 and HCC827ER cells. e SOD2 mRNA levels of HCC827, HCC827ER, and HCC827GR cells were detected by RT-PCR. f Western blot analysis of SOD2 and GPX4 protein expression in HCC827 cells, HCC827ER cells, and HCC827GR cells. g Analysis of GPX4 expression in lung adenocarcinoma patient tumors and normal tissues. The analysis was based on TCGA and GEO datasets. h Analysis of the association between the probability of overall survival (OS) of lung adenocarcinoma patients (n = 720) and their GPX4 gene expression profiles. The analysis was performed based on TCGA and GEO datasets. Low or high levels of GPX4 were defined as higher or lower than the median value of 720 patients. i Analysis of the association between the probability of overall survival (OS) of lung cancer patients (n = 1010) and their SOD2 gene expression profiles. The analysis was performed based on the TCGA dataset. Low or high levels of SOD2 were defined as higher or lower than the median value of 1010 patients. Data are presented as the mean ± SEM. *P < 0.05 vs Ctr.

Journal: Acta Pharmacologica Sinica

Article Title: NRF2-GPX4/SOD2 axis imparts resistance to EGFR-tyrosine kinase inhibitors in non-small-cell lung cancer cells

doi: 10.1038/s41401-020-0443-1

Figure Lengend Snippet: a, b Cell death was determined by flow cytometry as described in the “Methods”. The cells were exposed to 100 μM DFO for 24 h. (c) ROS were analyzed by flow cytometry as described in the “Methods”. d Western blot analysis of the anti-apoptosis protein BCL2 and the DNA damage biomarker γH2AX in HCC827 and HCC827ER cells. e SOD2 mRNA levels of HCC827, HCC827ER, and HCC827GR cells were detected by RT-PCR. f Western blot analysis of SOD2 and GPX4 protein expression in HCC827 cells, HCC827ER cells, and HCC827GR cells. g Analysis of GPX4 expression in lung adenocarcinoma patient tumors and normal tissues. The analysis was based on TCGA and GEO datasets. h Analysis of the association between the probability of overall survival (OS) of lung adenocarcinoma patients (n = 720) and their GPX4 gene expression profiles. The analysis was performed based on TCGA and GEO datasets. Low or high levels of GPX4 were defined as higher or lower than the median value of 720 patients. i Analysis of the association between the probability of overall survival (OS) of lung cancer patients (n = 1010) and their SOD2 gene expression profiles. The analysis was performed based on the TCGA dataset. Low or high levels of SOD2 were defined as higher or lower than the median value of 1010 patients. Data are presented as the mean ± SEM. *P < 0.05 vs Ctr.

Techniques: Flow Cytometry, Western Blot, Biomarker Discovery, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression

a Western blot analysis of NRF2, GPX4, and SOD2 protein expression. HCC827 cells were exposed to 5 μM KI696 for 24 h or 48 h, and HCC827ER cells were transfected with 20 nM siNRF2 or mock control for 48 h. b–e Cell viability was determined by CCK-8 assay, and cell growth curves were determined by IncuCyte. HCC827 cells were treated with 5 μM KI696, 100 nM TKIs, 40 nM RSL3, and 20 nM siNRF2 as indicated. f Analysis of the association between the probability of overall survival (OS) of lung cancer patients (n = 496) and their GPX4 and SOD2 gene expression profiles. The analysis was performed based on the TCGA dataset. Low or high levels of GPX4 and SOD2 were defined as those higher or lower than the median value of 496 patients. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Ctr.

Journal: Acta Pharmacologica Sinica

Article Title: NRF2-GPX4/SOD2 axis imparts resistance to EGFR-tyrosine kinase inhibitors in non-small-cell lung cancer cells

doi: 10.1038/s41401-020-0443-1

Figure Lengend Snippet: a Western blot analysis of NRF2, GPX4, and SOD2 protein expression. HCC827 cells were exposed to 5 μM KI696 for 24 h or 48 h, and HCC827ER cells were transfected with 20 nM siNRF2 or mock control for 48 h. b–e Cell viability was determined by CCK-8 assay, and cell growth curves were determined by IncuCyte. HCC827 cells were treated with 5 μM KI696, 100 nM TKIs, 40 nM RSL3, and 20 nM siNRF2 as indicated. f Analysis of the association between the probability of overall survival (OS) of lung cancer patients (n = 496) and their GPX4 and SOD2 gene expression profiles. The analysis was performed based on the TCGA dataset. Low or high levels of GPX4 and SOD2 were defined as those higher or lower than the median value of 496 patients. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Ctr.

Techniques: Western Blot, Expressing, Transfection, Control, CCK-8 Assay, Gene Expression

a–c Cell viability was determined by CCK-8 assay. HCC827ER, HCC827GR, and HCC827 cells were treated with 100 nM EGFR-TKIs and 20 nM siNRF2 for 72 h. d, e Cell viability was determined by CCK-8 assay. HCC827ER and HCC827GR cells were treated with 100 nM EGFR-TKIs and 20 nM siSOD2 for 72 h. f, g Cell viability was determined by CCK-8 assay. The resistant cells were treated with 80 nM RSL3 and every concentration of TKIs by triple dilution from 100 μM for 72 h. h, i Cell viability was determined by CCK-8 assay; HCC827GR, HCC827OR were treated with 10 μM ML385 and every concentration of TKIs by triple dilution from 100 μM for 72 h. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Ctr.

Journal: Acta Pharmacologica Sinica

Article Title: NRF2-GPX4/SOD2 axis imparts resistance to EGFR-tyrosine kinase inhibitors in non-small-cell lung cancer cells

doi: 10.1038/s41401-020-0443-1

Figure Lengend Snippet: a–c Cell viability was determined by CCK-8 assay. HCC827ER, HCC827GR, and HCC827 cells were treated with 100 nM EGFR-TKIs and 20 nM siNRF2 for 72 h. d, e Cell viability was determined by CCK-8 assay. HCC827ER and HCC827GR cells were treated with 100 nM EGFR-TKIs and 20 nM siSOD2 for 72 h. f, g Cell viability was determined by CCK-8 assay. The resistant cells were treated with 80 nM RSL3 and every concentration of TKIs by triple dilution from 100 μM for 72 h. h, i Cell viability was determined by CCK-8 assay; HCC827GR, HCC827OR were treated with 10 μM ML385 and every concentration of TKIs by triple dilution from 100 μM for 72 h. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Ctr.

Techniques: CCK-8 Assay, Concentration Assay

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